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KMID : 1007520080170020434
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Food Science and Biotechnology
2008 Volume.17 No. 2 p.434 ~ p.437
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Potential Effect of Monascus-fermented Soybean Extracts on Alkaline Phosphatase Activity of Human Osteoblast-Like Cells
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Pyo Young-Hee
Kwon Mi-Ja
Kim In-Ho
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Abstract
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The aim of this study was to investigate whether Monascus-fermented soybean extracts (MFSE) containing natural estrogen-like compounds such as isoflavones and mevinolins has potential effects on human osteoblast-like SaOS2 cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and alkaline phophatase (ALP) assaies. MFSE exerted biphasic dose-dependent effect; stimulating osteoblastic activity at low concentrations and inhibiting SaOS2 cells viability at high concentrations. At 10-8-10-4 mg/mL, MFSE is not only non-cytotoxic but also induced comparatively high ALP activity on SaOS2 cells. ALP activity (%) significantly increased (220.1%, p<0.05) when SaOS2 cells were treated with MFSE at a concentration of 10-5 mg/mL, whereas slowly increased (185.6%, p<0.05) in unfermented soybean extracts (UFSE) at 10-3 mg/mL. The potentially greater ALP activity of MFSE compared to the UFSE might partially be caused by its mevinolin, which was derived from the soybean during Monascus-fermentation. Our findings indicate that supplementation of MFSE may accelerate the speed of intracellular ALP synthesis by the bone cells when provided at optimal dosages.
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KEYWORD
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Monascus-fermentation, soybean, human osteoblast-Like cell, alkaline phophatase activity, mevinolin, isoflavone
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